These findings coincide with previous studies showing that Fas is involved in apoptosis of embryonic cortical neuroblasts and in the development of fetal brain. Differentiation and proliferation are two sides of the same coin. Clonidine hydrochloride Although whether grafted NSCs mainly specialize into neuronal or glial lineage remains controversial, excessive astrogliosis may hamper axon outgrowth and reduce the function of NSCs. To determine whether harvesting media exposure could influence NSC differentiation, we identified postmitotic cells that differentiated from NSCs. We found that PBS and ACSF do not affect cell differentiation significantly. However, exposure to Saline does not facilitate neuronal differentiation, suggesting that Saline exposure may attenuate in vitro neurogenesis probably due to the low pH. As expected, neurospheres unaffected by long-term harvesting media exposure induced proliferation decline are also resist to neuronal differentiation deficiency after Saline exposure. Our results indicate that in vitro differentiation of NSCs could be at least partly dependent on the type of harvesting media, although the signaling pathways mediating the harvesting media exposure-associated modulation of differentiation of NSCs remain to be determined. Poor cell quality might lead to low viability and low levels of cell engraftment after transplantation. To evaluate whether harvesting media exposure affects morphological and functional recovery, treated EGFP-NSCs were grafted by in situ BMS-740808 transplantation into the injured cortex. A considerably lower number of GFP + cells migrate to the damaged site and integrated with the host tissue in the sections of low-scoring animals in various groups than in the sections of higher-scoring animals, indicating that the functional test score parallels the number of GFP + cells in the damaged site. Furthermore, the graft-derived cells in low-scoring animals bear obviously weaker competence of synaptogenesis than those in higher-scoring animals. Of note, the harvesting media-exposed NSC groups have fewer GFP + cells as well as weaker synapse formation of graft-derived cells and low scoring animals than the PCM-exposed NSC group.