Transcription factors are particularly important for fiber cellinitiation. GhMYB25,GhMYB25-like,and GhHD1 were previously identified as regulators of lintfiber initiation, and silencing of GhMYB25 in cotton led to delayed fiber initiation and formation of shorter fibers, and overexpression of GhHD1 stimulated fiber initiation, while silencing delayed this process. In addition to transcription factors, Triamterene phytohormones play key roles in plant development and have been well-studied. At anthesis, ovules initiate fiber development in cultures supplemented with both IAA and GA3, whereas ABA, ethylene, and cytokine insinhibit fiber development. Numerous genes involved in IAA, GA, and BR biosynthesis and signalling pathways have been cloned and confirmed to be associated with the development of fiber initiation. Additionally, genes involved in calcium signaling pathways and calcium and ROS homeostasis play crucial regulatory roles during fiber cell initiation and differentiation, and the Susgene encoding sucrose synthase is also involved. In this study, the Fisher��s exact test was finished to XinWX and XinFLM at three time points during fiber initiation development, and transcription factors such as ERFs, MYBs, WRKYs and Zinc-finger proteins were indicative of a positive regulatory effect on early fiber development, indicating important roles in the fiber initiation. Tubastatin A Further, in hormone-associated metabolic pathways, GO terms in JA pathway were particularly enriched, especially AOCs, the key enzymes in JA bio synthesis, which suggests that JA is related to progression of fiber initiation. Additionally, ��Lipidtransport�� and ��asparagine biosynthetic process�� was also enriched during fiber initiation development. LTPs transport lipids from the endoplasmic reticulum to the plasma membrane and the cell exterior, and are known to be associated with fiber elongation. LTPs are usually activated during the early stages of fiber development, and expression levels peaked at around 10DPA.Arfinineis the predominant nitrogen storage unit in cotton and accounts for at least25% of total stored nitrogen, and the majority of nitrogen transported between cotyledons and the growing axis is via the asparagine pathway.