A short mobile phase and a long, intermittent stationary phase that may represent of the total journey time are iterated as the neurofilament is transported. Once the peptide reaches the proximal end of the dendrite it must be transported to a cholinergic synapse with nAChR receptors for an effect to occur. Its apparent localisation in the perinuclear region of neuronal cell bodies suggests it may become associated with the trans-Golgi network. It is here that neuropeptide precursors and accessory enzymes required for their into synaptic vesicles by a specific vesicular acetylcholine transporter. Both proteins are abundantly expressed in the nerve ring. Therefore the nerve ring is a site of nAChRs that the peptide does access after uptake by the dye-filling neurons. The peptide is also present immediately posterior to the nerve ring and in the region of the lateral ganglia of C. elegans where the cell bodies of both the dye-filling neurons and the interneurons to which they connect occur. Three-dimensional culture of muscle cells has been proposed to provide a more physiological in vitro model of muscle growth and differentiation than routine 2D cultures, thus providing an advanced in vitro modelling of skeletal muscle. In addition, the creation of skeletal muscle tissue using engineering methods has tremendous potential for the treatment of lost or severely damaged muscles. The biomaterial scaffold plays a key role in most tissue engineering strategies. To guide the organization, growth, and differentiation of cells in tissue engineered constructs, the scaffold should be able to provide a physical support for the cells, and the chemical and biological cues necessary for the formation of a functional tissue. A number of synthetic materials have been developed to provide well controlled and reproducible 3D support of myocyte culture. Alternatively, natural hydrogels present important advantages for engineering functional muscle, primarily because of their higher Veratramine capacity to provide appropriate adhesion sites for the cells. In particular, fibrin is an attractive matrix for stem cell differentiation and muscle tissue engineering notably because it can interact with integrins and has the capacity to bind specifically many growth factors. A recent study indicates that fibrin gel improves the survival of transplanted myoblasts, probably through cell-matrixanchorage signalling. Interestingly, fibrin supports the parallel orientation of myotubes under directed Halothane mechanical constraints, and thus replicates some crucial aspects of the native skeletal muscle cell patterning.