ROS scavenging enzymes in plants include superoxide dismutase, peroxidases, catalase, guaiacol peroxidase, ascorbate peroxidase, dehydroascorbate reductase and glutathione reductase. A large body of evidence has demonstrated that the antioxidant systems play important roles in protecting plants against oxidative damage induced by cold stress. The 5-aminolevulenic acid is a key precursor in the biosynthesis of all porphyrins compounds such as chlorophyll, heme and phytochrome. A number of reports show that exogenous ALA improves the growth and yield of a number of plants by enhancing chlorophyll contents and the rate of photosynthesis. It is also known that ALA in low concentration regulates key physiological processes associated with plant growth under various abiotic and biotic stresses, including low or high temperature, salinity, drought and heavy metals. In contrast, high levels of ALA can promote enhanced production of ROS, thereby enhancing oxidative stress in plants. These results suggest that ALA has a great application potential in agricultural production as a new non-toxic endogenous Fomepizole substance. Elymus nutans Griseb., a perennial cool-season grass, is distributed in the north, northwest and southwest regions, especially on the Qinghai-Tibetan Plateau from 3,000 to 5,000 m in China. E. nutans has been traditionally used as typical native forage and has often been collected and dried as long cool season. Recently, it has been widely planted in cultivated pastures in alpine areas, owing to its high adaptability, good nutrition, high yield and good resistance to cold, drought and biotic stress. Thus, an investigation of seed germination in low temperature is important to wild E. nutans establishment at high altitude in Qinghai-Tibetan plateau. Chen and Jia reported E. nutans also plays a pivotal role in animal husbandry and environmental sustenance in China. However, to date, no specific information is available Carbosulfan regarding the effects of ALA on cold stress resistance of E. nutans seeds. Moreover, further studies are required to elucidate the mechanism of how ALA application could regulate specific metabolic reactions to achieve enhanced resistance in seeds to temperature stress.