In addition to the potential Tulathromycin B effects of the uniform core of metabolites as the primary determinants of the success of the host-parasite relationship between triatomines and T. cruzi, variable metabolites may act as secondary determinants and are likely critical to the course of the host-parasite relationship. These secondary determinants may play their roles through direct actions as well as synergistic or antagonistic effects of multiple metabolites whose proportions may vary according to the insect host. It is well known that different T. cruzi strains do not have the same developmental success in different triatomine species and different ecological conditions; T. cruzi virulence varies according to the geographic location. All three species tested here are good vectors for T. cruzi. Thus, comparing the metabolome outlined here to that of triatomines with poor T. cruzi transmission efficiency would most likely aid in identifying factors involved in the development of T. cruzi. Interestingly, compounds like eicosanoids, which may play a role in metacyclogenesis, varied quantitatively among triatomine species in our study. Because the developmental success of a T. cruzi strain in its invertebrate host may depend on the triatomine species and the intestinal microbiota depends on the triatomine species, we suggest that a tripartite relationship is critical to the interactions between triatomines and T. cruzi. In addition, T. cruzi can also affect the microbiota composition upon triatomine infection, which, in turn, may affect the metabolic 
composition of triatomine feces, with significant feedback consequences on the parasite. Altogether, these observations suggest that a specific homeostatic system controlling the Estradiol Benzoate epidemiology of Chagas disease may exist. Therefore, it is necessary to determine how the three biological components of the triatomine-T. cruzi host-parasite interaction may affect the developmental success of the parasite. As such, this work is the first attempt to understand the complexity of the fecal metabolome in triatomines as a path for further investigations of triatomine vector competence for specific T. cruzi strains. Our work has opened many avenues for investigations concerning the role of individual metabolites in the interactions among T. cruzi, its triatomine vectors and the vertebrate host. Knowledge of these interactions is likely to generate new ways to understand the factors influencing parasite proliferation and spread as well as methods to control Chagas disease. The dosage compensation is an essential process that equalizes the expression levels of Xchromosomal genes between males and females. The complex is a model for studying chromosome-wide transcription regulation by histone hyperacetylation. It is actually known that the histone acetyltransferase MOF is tightly regulated by MSL1 and MSL3, and it even intervenes in the outcome of autophagy. In Drosophila, the complex also involves two functionally redundant non-coding RNAs ; in mammals, no associated RNA has been yet identified, and there are two MOF-MSL complexes. Both complexes are important for most of the histone 4 K16 acetylation in cells, for gene expression, cell-cycle regulation, DNA-damage repair and other regulatory processes in the nucleus. In addition to the MSL complex, MOF also resides in an NSL complex, that plays an important role in genome-wide chromatin modification and transcriptional regulation. The human 614-residue-long and the 1039-residue-long Drosophila MSL1s are predicted to contain no globular domains. Both, the scaffold of the MSL complex and dimerization of MSL1 coiled-coil region with the MSL2 protein are essential for male X-chromosome regulation in Drosophila.