Second, clinical diagnosis was a feature of the present study: this limits the reliability of the diagnosis of depression and other possible comorbid psychiatric disorders. Use of structured diagnostic interview schedules may increase the reliability and validity of diagnoses. Third, given the size of our samples, we could not stratify patients who had attempted suicide by the severity of their suicide attempts. Fourth, the patients included in our analysis had different baseline characteristics compared with excluded patients. Included patients were more likely to be female and employed, and they had lower baseline scores on the CGI-S, HAMD, BDI, and SSI-B and higher scores on the SOFAS and WHOQOL compared with excluded patients. In a previous study investigating TCI in Temozolomide remitted depressive patients, socio-demographic variables, including education, age, and gender, did not significantly contribute to TCI scores. However, the inclusion of patients with milder depressive symptoms and a higher degree of functioning, may limit the generalizability of the results of this study. Furthermore, detailed information pertaining to treatment was not included. Multiple, uncontrolled treatments can limit the interpretative validity of results. In a previous study, persistent suicidality was associated with treatment with selective serotonin reuptake inhibitors, a higher baseline SSI-B score and no HAMDor HAMA-indexed remission episodes. The present results should be interpreted in this context. Finally, certain TCI scores are likely to change over time. We could not investigate changes in TCI scores in an attempt to limit this bias because TCI was only administered at week 12. In conclusion, we did not observe differences among patients who attempted suicide and those with and without suicidal ideation in any of the dimensions of the TCI, with the exception of ST and SD. Longitudinal studies that include younger age groups are required to disentangle the personality profile of individuals with high suicidality from underlying psychopathology. The findings of the present study may further the delineation of this complex phenotype. Cyanobacterial blooms and the production of secondary metabolites cyanotoxins represent a serious public health hazard to humans and animals worldwide since the cyanotoxins can be accumulated in aquatic organisms and transferred to higher trophic levels. One of the most frequently studied cyanotoxins is the cyclic heptapeptide hepatotoxins called microcystins due to their wide distribution and high toxicity. Up to now, more than 80 analogues of MCs have been identified, with microcystin-LR being the most common and toxic. Studies demonstrated that the mechanism of MCs toxicity is the potent inhibition of serine/threonine- specific protein phosphatases 1 and 2A, which then leads to the hyperphosphorylation of key control proteins that regulate tumor promotion or apoptosis.