Both of these proteins contain predicted Ig-like domains that are perfectly compatible with high content of beta structure. The structure of other proteins was mixed. Stability studies were performed by monitoring the CD signal of protein solutions supplemented with increasing concentrations of denaturant. The least stable were gp23 and Soc, having GdnHCl K of 1.16 M and 0.66 M, respectively. Hoc protein was more stable. Interestingly, gp24 expressed two phases of denaturation, which corresponds well with its structure and existence of two separate domains, a smaller domain A and a larger, more stable domain B. The smaller domain of gp24 protein consists of only three beta strands and one alpha helix, and has no contacts with the larger domain. Thus we assume that out of the two domains the smaller one has lower stability. Interestingly, two essential capsid proteins, gp23 and gp24, which are known to be homologous, differ substantially in their stability in vitro. The four proteins characterised in this work make up the greater part of the T4 phage capsid surface, thus being important targets for immunological studies. Although not involved directly in the processes of infection and lysis of the host cell, they may play a key role for phage survival in its environment, mediating complex interactions of the phage with external factors. Since T4 phage infects E. coli, its propagation may often be connected with mammalian organisms. Phage impact on human and NVP-BKM120 customer reviews animal bodies, which is mediated mostly by phage capsid proteins, is still poorly recognised, while it might be decisive for future phage applications: implementation of phage therapies, phage-based vaccines or drug carriers and other medical solutions. Sepsis is a state of sustained infection that results in a severe systemic inflammatory response and, possibly, shock. Despite significant improvements in critical care medicine during recent decades, sepsis remains as one of the leading causes of mortality in the intensive care unit. Numerous studies have attempted to identify biomarkers to predict sepsis mortality, including serum Creactive protein, Procalcitonin levels, and Acute Physiology and Chronic Health Evaluation II scores and Sequential Organ Failure Assessment scores. To our knowledge, only miR-223 and miR-146a have shown diagnostic value for sepsis and only one plasma miRNA, miR-150, was identified as a potential biomarker for sepsis prognosis. As is well known, sepsis is a complex syndrome that involves multiple organs and tissues, and not only leukocytes, and a single biomarker is not sufficient to reflect the severity of sepsis. Thus, a panel of biomarkers is needed to evaluate sepsis prognosis. There have been no studies that used genome-wide screening of serum or plasma for sepsis prognosis or mortality from sepsis. Numerous methods have been used to detect the genome-wide expression profiles of miRNAs, such as Exqion miRCURY LNA array technology, the Illumina BeadChip platform, the Febit automated Geniom Real Time Analyzer platform, and Affymetrix GeneChip miRNA array technology.