{"id":218,"date":"2018-12-21T08:08:21","date_gmt":"2018-12-21T07:08:21","guid":{"rendered":"http:\/\/mapkinhibitorlibrary.com\/?p=218"},"modified":"2022-01-17T11:38:10","modified_gmt":"2022-01-17T02:38:10","slug":"interesting-highlight-toll-like-receptors-typical-role","status":"publish","type":"post","link":"http:\/\/mapkinhibitorlibrary.com\/index.php\/2018\/12\/21\/interesting-highlight-toll-like-receptors-typical-role\/","title":{"rendered":"It is interesting to highlight that some Toll-like receptors with a typical role"},"content":{"rendered":"

IRF3 and IRF7 were also affected by the vaccine at the same time point. Moreover, some genes related with the activity of the transcription factor NF-kappa-B, such as the activator IKKbeta or the NF-kappa-B inhibitor zeta, were significantly down-regulated by pMCV1.4-G860. TLR2 recognize other viral components such as envelope glycoproteins and, although no significant up-regulations were observed for this gene after vaccination, the heat map reflects a slight induction. TLR2 activation induces apoptosis through a FADD\/Caspase 8 pathway and both genes appeared overrepresented at 72 h, revealing a possible stimulation of the TLR pathway via TLR2 and ultimately, inducing apoptosis. With regard to the viral challenge, it is interesting to highlight that some Toll-like receptors with a typical role in bacterial component detection have been found to be strongly regulated after VHSV infection, and this Acetohydroxamic acid<\/a> induction could suggest a novel role of these receptors in the recognition of viral components. A sequence annotated as TLR13 was also modulated at 24 h; there is little information about the function of this receptor and the nature of their ligands remains still poorly Notopterol<\/a> understood, but there are evidences about the recognition of bacterial rRNA as well as vesicular stomatitis virus by TLR13. As was expected, the typical viral-recognition receptors TLR3 and TLR8 were significantly up-regulated after VHSV challenge. Focusing the attention in the downstream signalling components, a pronounced induction of several proteins was observed. Some molecules were also found to be down-regulated, including inhibitors of the transcription factor NF-kappa-B as well as numerous molecules implicated in their activation, possibly due to the maintenance of equilibrium in the NF-kappa-B activity. On the other hand, VHSV infection in vaccinated fish revealed a completely different pattern, even TLR2 and TLR5 were found to be significantly downregulated at 24 h and the other TLRs were not affected by the viral infection. As a consequence, the induction of downstream proteins was practically suppressed or down-regulated and only in the case of IRF3 and IRF7 significant up-regulations were detected at 24 h, with a return to the basal levels at 72 h.<\/p>\n","protected":false},"excerpt":{"rendered":"

IRF3 and IRF7 were also affected by the vaccine at the same time point. Moreover, some genes related with the activity of the transcription factor NF-kappa-B, such as the activator IKKbeta or the NF-kappa-B inhibitor zeta, were significantly down-regulated by pMCV1.4-G860. TLR2 recognize other viral components such as envelope glycoproteins and, although no significant up-regulations … Continue reading It is interesting to highlight that some Toll-like receptors with a typical role<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"http:\/\/mapkinhibitorlibrary.com\/index.php\/wp-json\/wp\/v2\/posts\/218"}],"collection":[{"href":"http:\/\/mapkinhibitorlibrary.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/mapkinhibitorlibrary.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/mapkinhibitorlibrary.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/mapkinhibitorlibrary.com\/index.php\/wp-json\/wp\/v2\/comments?post=218"}],"version-history":[{"count":1,"href":"http:\/\/mapkinhibitorlibrary.com\/index.php\/wp-json\/wp\/v2\/posts\/218\/revisions"}],"predecessor-version":[{"id":219,"href":"http:\/\/mapkinhibitorlibrary.com\/index.php\/wp-json\/wp\/v2\/posts\/218\/revisions\/219"}],"wp:attachment":[{"href":"http:\/\/mapkinhibitorlibrary.com\/index.php\/wp-json\/wp\/v2\/media?parent=218"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/mapkinhibitorlibrary.com\/index.php\/wp-json\/wp\/v2\/categories?post=218"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/mapkinhibitorlibrary.com\/index.php\/wp-json\/wp\/v2\/tags?post=218"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}